Actividad antiinflamatoria y antioxidante de Merremia Umbellata (L.) Hallier f.
Actividad antiinflamatoria y antioxidante de Merremia Umbellata (L.) Hallier f.
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Introducción: Merremia umbellata es ampliamente utilizada en la medicina popular para tratar múltiples enfermedades, incluyendo aquellas que cursan con inflamación.
Objetivos: evaluar el efecto antiinflamatorio y antioxidante del extracto etanólicototal obtenido de las hojas de Merremia umbellata.
Métodos: el material vegetal fue extraído por maceración con etanol. La actividad antiinflamatoria se evaluó utilizando el modelo murino in vivo de edema auricular inducido por 13-acetato de 12-orto-tetradecanoilforbol (TPA), determinando la actividad de la enzima mieloperoxidasa (MPO). Adicionalmente se determinó la inhibición de la producción de óxido nítrico (NO) en macrófagos RAW 264.7 estimulados con lipopolisacarido (LPS). Mientras que la actividad antioxidante se determinó aplicando los métodos espectrofotométricos de captación de los radicales libres DPPH (2,2-difenil-1-picrilhidrazilo), ABTS (sal diamónica del ácido 2,2’azinobis-(3-etilbenzotiazolin 6- sulfónico), NO y H2O2 (peróxido de hidrógeno).
Resultados: el extracto de Merremia umbellata disminuyó el edema y la actividad de la enzima MPO en orejas de ratones inflamadas con TPA, así como la producción de NO en macrófagos activados. Además presentó significativa actividad captadora de los radicales libres DPPH, ABTS y H2O2.
Conclusiones: se demostró actividad antiinflamatoria y antioxidante en el extracto etanólico total de Merremia umbellata. Rev.Cienc.Biomed. 2013;4(1):13-19
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